TITRATION OF TETANUS ANTITOXIN BY PASSIVE HEMAGGLUTINATION

Factors affecting the passive hemagglutination titration: dilution loops, titration trays, vibration, diluents.

Serial dilution with Takatsy loops resulted in exaggerated passive hemagglutination titers with most of the anti-bovine serum albumin sera tested. It appears that certain types of agglutinins adhere to the loop surface and are released only gradually. This adherence, or carry-over effect, was prevented by presoaking loops in gelatin or gelatin-rabbit serum-albumin solutions. Hemolysins did not ...

Human tetanus antitoxin.

Tetanus Antitoxin from Intraocular Transplants **

Evidence for antibody formation by various cells, tissues, and organs has come mainly from studies employing the following methods: (i) extracts of tissue cultures,'l (ii) cannulization of afferent and efferent lymph,"'X (iii) extracts of cells, tissues, and organs,"' (iv) extirpation of the spleen," and (v) roentgen-radiation of animals with lead-protected mobilized spleens.' Although the repo...

The Destruction of Tetanus Antitoxin by Chemical Agents.

It is only to be regretted that this step was not taken earlier, for the large number of idle bulls now in the herd is both a detriment to that body and a positive loss of once good skins. This menace in the growth of the herd has been repeatedly pointed out (Clark, 1914, 1916, 1917; Osgood, Preble, and Parker, 1915) to those having the matter in charge. The last column in table 1 contains the ...

Tetanus antitoxin titres in military personnel.

A study was made of the titres of tetanus antitoxin in the sera of 186 recruits and 886 serving personnel. All were found to have titres of more than 0.01 IU per ml, the titre regarded as sufficient to provide protection from tetanus. The percentage of recruits with tetanus antitoxin titres less than 0.1 IU per ml was significantly greater than the percentage of serving personnel with antitoxin...